Subject: P{GawB}109(2)80, P{GawB}477 and P{GawB}221
I received the following note from Susan Younger, University of California
at San Francisco (6/3/5).
Gal4109-2-80 is from our Gal4 screen where we hopped a P{w+mW.hs=GawB}
from the X onto the second or the third starting with either (1) 7020
P{GawB}109C1 or (2) 7021 P{GawB}112A as indicated by the first numbers, 109
or 112. Bloomington has several others from this screen all starting with
109. The second number, as in 109-2, indicated which autosome carries the
insertion, \-2 or \-3. As for 109-2-80, I mapped this insertion by chromosome
in situ to 25D and it has been published by our lab in several
references. It already has a flybase ID: FBti0015554.
The other two Gal4 lines, 221 and 477, are from a screen done in Ulrike
Heberlein's lab. They started with the same Gal4 X chromosome lines that we
did, 109 or 112, a P{w+mW.hs=GawB}. We were able to screen through some
of them and identified 221 and 447 as lines expressed in the dendrites. We
have published both of them in a paper by Wes Grueber, Cell 2003
112(6):805-818. They are in flybase:
P{GAL4}477 FBti0026455 Insertion is on the 2nd chromosome.
P{GAL4}221 FBti0026457 Insertion is on the 3rd chromosome.