FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Insertion: Dmel\P{GawB}221
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General Information
Symbol
Dmel\P{GawB}221
Species
D. melanogaster
Name
FlyBase ID
FBti0187661
Feature type
transposable_element_insertion_site
Also Known As
221-Gal4, 2-21-GAL4, Gal42-21, Gal4221, 2-21
Description
Inserted Element
P{GawB}
LINE ID
Affected gene(s)
Scer\GAL4
Viability / fertility
Associated allele(s)
Scer\GAL4221
Stock availability
None publicly available
Genomic Location
Chromosomal Location
3
Sequence Location
Target / Docking site
Member of Large Scale Dataset(s)
Dataset
Description
Detailed Mapping Data
Chromosome (arm)

3

(Younger, 2005.6.3)
Sequence Location
Orientation
Cytological location (computed by FlyBase)
Cytological location (reported)
Insertion into Natural transposon
Comments concerning location
Sequence Data
Flanking sequence
Inserted Element
Construct
P{GawB}
(Younger, 2005.6.3)
Size (Kb)

11.279

Component Allele(s)
Scer\GAL4wB
w+mW.hs
Molecular map
Affected Gene(s)
Phenotypic Data
Expression Data
Reporter Expression
distribution deduced from reporter
Stage
Tissue/Position (including subcellular localization)
Reference
embryonic stage 16 -- late third instar larval stage
larval multidendritic class I neuron
(Parrish et al., 2009)
distribution deduced from reporter (Gal4 UAS)
Stage
Tissue/Position (including subcellular localization)
Reference
embryonic stage 17 -- third instar larval stage
larval multidendritic class I neuron
(Lin et al., 2015)
larval stage
larval dorsal multidendritic neuron ddaE
(Xu et al., 2008)
larval dorsal multidendritic neuron ddaF
(Xu et al., 2008)
abdominal 3 dorsal multidendritic neuron ddaD
(Sun et al., 2012)
abdominal 3 dorsal multidendritic neuron ddaE
(Sun et al., 2012)
larval multidendritic class I neuron
(Cheng et al., 2010)
bipolar dendrite neuron
(Cheng et al., 2010)
second instar larval stage
abdominal posterior ventral multidendritic neuron vdap
(Grueber et al., 2003)
third instar larval stage
bipolar dendrite neuron | subset
(Hughes and Thomas, 2007)
larval multidendritic class I neuron | subset
(Hughes and Thomas, 2007)
abdominal dorsal bipolar neuron dbp
(Hughes and Thomas, 2007)
abdominal dorsal multidendritic neuron ddaD
(Hughes and Thomas, 2007)
abdominal dorsal multidendritic neuron ddaE
(Hughes and Thomas, 2007)
abdominal posterior ventral multidendritic neuron vdap
(Hughes and Thomas, 2007)
larval brain | restricted
(Hughes and Thomas, 2007)
larval ventral nerve cord | restricted
(Hughes and Thomas, 2007)
larval multidendritic class I neuron
(Hu et al., 2017, Tsubouchi et al., 2012)
larval dorsal multidendritic neuron ddaC
(Hattori et al., 2013)
larval dorsal multidendritic neuron ddaD
(Hattori et al., 2013)
larval dorsal multidendritic neuron ddaE
(Hattori et al., 2013)
adult stage
abdominal 5 dorsal multidendritic neuron ddaD
(Shimono et al., 2009)
abdominal 6 dorsal multidendritic neuron ddaD
(Shimono et al., 2009)
abdominal dorsal multidendritic neuron ddaC
(Shimono et al., 2009)
abdominal anterior ventral multidendritic neuron vdaa
(Shimono et al., 2009)
Additional Information
Statement
Reference

Drives expression in class 1 dendritic arborizing neurons.

(FlyBase Curators, 2017)

ScerGAL4221 drives expression in class I dendritic arborizing neurons starting at embryonic stage 17; while ScerGAL498b does not begin driving expression until the first larval instar.

(Lin et al., 2015)

ScerGAL42-21 drives expression in some neurons in the PNS including class I dendritic arborizing neurons (ddaD, ddaE and vdap) and in the longitudinal bipolar dendrite neurons (dbp). Labelling is also seen in a few cells in the brain and ventral nerve cord.

(Hughes and Thomas, 2007)
 
Marker for
Reflects expression of
Reporter construct used in assay
Mmus\Cd8aUAS.cLa.EGFP
(Tsubouchi et al., 2012, Parrish et al., 2009, Shimono et al., 2009, Xu et al., 2008, Hughes and Thomas, 2007, Grueber et al., 2003)
ctUAS.cPa
(Grueber et al., 2003)
Hsap\CUX1UAS.cLa
(Grueber et al., 2003)
mir-124UAS.cXa
(Xu et al., 2008)
nompCL.UAS
(Cheng et al., 2010)
Avic\GFPMhc-C110
(Hughes and Thomas, 2007)
Data on Genetic Line
Line ID
Origin as a multiple insertion line
Progenitor(s) within the Genome
Related Aberration or Balancer
Aberration
Balancer
Stocks (0)
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (22)
Reported As
Symbol Synonym
2-21
(Babic et al., 2015, Zhang et al., 2013, Shimono et al., 2009, Hughes and Thomas, 2007)
2-21 gal4
(Palavalli et al., 2021)
2-21-GAL4
(Krämer et al., 2023, Zeng et al., 2021, Mauthner et al., 2014, Tsubouchi et al., 2012, Hwang et al., 2007)
2-21-Gal4
(Zhang et al., 2020, Held et al., 2019, Hu et al., 2017, Sears and Broihier, 2016, Yalgin et al., 2015, Mazock et al., 2010, Parrish et al., 2009, Zheng et al., 2008)
2-21Gal4
(Nagel et al., 2012)
221-GAL4
(Rao et al., 2016)
221-Gal4
(Kumar et al., 2026, Mahata et al., 2025, Thyagarajan et al., 2025, Niu et al., 2023, Swope et al., 2022, Feng et al., 2021, Li et al., 2021, Nye et al., 2020, Weiner et al., 2020, Feng et al., 2019, Hao et al., 2019, Chen et al., 2016, Jegla et al., 2016, Weiner et al., 2016, Lin et al., 2015, Lo et al., 2015, Yang et al., 2015, Janssens et al., 2014, Nguyen et al., 2014, Stone et al., 2014, Chen et al., 2012, Hill et al., 2012, Kim et al., 2012, Song et al., 2012, Nguyen et al., 2011, Tao and Rolls, 2011, Mattie et al., 2010)
221-gal4
(Kwon et al., 2018)
221:Gal4
(Hertzler et al., 2024)
C-I GAL4
(Iyer et al., 2013)
CI-Gal4
(Turner et al., 2016)
GAL4-2-21
(Huang et al., 2011)
GAL42-21
(Hattori et al., 2007)
GAL4221
(Iyer et al., 2013)
Gal42-21
(Wong et al., 2013, Kirilly et al., 2011, Kirilly et al., 2009, Dimitrova et al., 2008, Jinushi-Nakao et al., 2007)
Gal4221
(Tran et al., 2015, Lu et al., 2009, Xu et al., 2008, Sweeney et al., 2006, Sweeney et al., 2006)
Gal4[2-21]
(Tsuyama et al., 2017)
P{GAL4}2-21
(Ye et al., 2004, FlyBase, 1992-)
P{GAL4}221
(Grueber et al., 2003, FlyBase, 1992-)
P{GawB}2-21
(FlyBase, 1992-)
P{GawB}221
(Younger, 2005.6.3, FlyBase, 1992-)
bd/I-Gal4
(Cheng et al., 2010)
Name Synonyms
Secondary FlyBase IDs
  • FBti0128084
  • FBti0026457
  • FBti0040394
References (86)