FlyBase Allele Report: Dmel\nompC[L.UAS]

General Information

Symbol

Dmel\nompC^L.UAS

Species

D. melanogaster

Name

FlyBase ID

FBal0246938

Feature type

allele

Associated gene

Dmel\nompC

Associated Insertion(s)

Carried in Construct

P{UAS-nompC.L}

Also Known As

UAS-nompC

Key Links

Transgenic product class

wild_type

(Cheng et al., 2010)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Cheng et al., 2010)

Mutagen

in vitro construct

(Cheng et al., 2010)

Progenitor genotype

Carried in construct

P{UAS-nompC.L}

Cytology

Description

UAS regulatory sequences drive expression of the longer 1732 amino acid residue nompC isoform.

(Cheng et al., 2010)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

nompC

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neurophysiology | adult stage, with Scer\GAL4^GH298

(Gong et al., 2013)

abnormal neurophysiology | adult stage, with Scer\GAL4^{kra-krasavietz}

(Gong et al., 2013)

abnormal neurophysiology | third instar larval stage, with Scer\GAL4^ppk.PH

(Yan et al., 2013)

Phenotype Manifest In

adult antennal lobe local neuron, with Scer\GAL4^GH298

(Gong et al., 2013)

adult antennal lobe local neuron, with Scer\GAL4^{kra-krasavietz}

(Gong et al., 2013)

Detailed Description

Statement

Reference

Expression of nompC^L.Scer\UAS under the control of Scer\GAL4^GH298 in antennal lobe interneurons renders these interneurons sensitive to mechanical stimulation (these neurons show no mechanosensitivity in wild-type controls). Inward currents are recorded at the onset and the end of the stimulus in the mutant interneurons. The latency of the major 'on' current is less than 5 ms and the amplitude correlates with the distance of stimulating probe displacements.

Expression of nompC^L.Scer\UAS in interneurons under the control of Scer\GAL4^{kra-krasavietz} causes these interneurons to become sensitive to mechanical stimulation.

(Gong et al., 2013)

Unlike in wild type, class IV dendritic arborization neurons expressing nompC^L.Scer\UAS under the control of Scer\GAL4^ppk.PH produce a burst of action potentials and an increase in internal calcium level in response to touch stimulation.

(Yan et al., 2013)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescues

Scer\GAL4^nompC.PC/nompC^L.UAS rescues nompC¹/nompC³

(Gong et al., 2013)

Scer\GAL4^19-12/nompC^L.UAS rescues nompC¹/nompC³

(Yan et al., 2013)

Scer\GAL4^nompC.PC/nompC^L.UAS rescues nompC¹/nompC³

(Cheng et al., 2010)

Scer\GAL4^5-40/nompC^L.UAS rescues nompC¹/nompC³

(Cheng et al., 2010)

Scer\GAL4^21-7/nompC^L.UAS rescues nompC¹/nompC³

(Cheng et al., 2010)

nompC^L.UAS/Scer\GAL4²²¹ rescues nompC¹/nompC³

(Cheng et al., 2010)

Comments

Expression of nompC^L.Scer\UAS under the control of Scer\GAL4^nompC.PC rescues the reduced locomotion of nompC¹/nompC³ adults.

(Gong et al., 2013)

Expression of nompC^L.Scer\UAS under the control of Scer\GAL4^19-12 rescues the touch sensitivity defects seen in nompC¹/nompC³ mutant larvae. The defects in behavior, action potential frequency and calcium response are all rescued.

(Yan et al., 2013)

The lethality of nompC¹/nompC³ larvae is rescued by expression of nompC^L.Scer\UAS under the control of Scer\GAL4^nompC.PC. The reduced crawling speed and increased stride duration seen in nompC¹/nompC³ larvae is also rescued.

The reduced crawling speed and increased stride duration of nompC¹/nompC³ larvae is rescued by expression of nompC^L.Scer\UAS under the control of either Scer\GAL4^5-40, Scer\GAL4^21-7 or Scer\GAL4^2-21.

The walking defects seen in nompC¹/nompC³ adults are rescued by expression of nompC^L.Scer\UAS under the control of either Scer\GAL4^21-7 or Scer\GAL4^nompC.PC.

(Cheng et al., 2010)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

nompC^L.Scer\UAS

nompC^L.UAS

Name Synonyms

Secondary FlyBase IDs

References (5)

Report Sections

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