Subject: Helping FlyBase: EDRC-CC13 Dear Margit, We are currently curating the abstracts for the 19th (Eger) European Drosophila Research Conference, for FlyBase. I am writing in connection with your abstract: Genetic analysis of the anaphase promoting complex in Drosophila melanogaster. You mention gene symbols that are new to FlyBase, APC4, APC7 and cdc23. Do you know which of the Genome Project CG annotations your genes correspond to? All the CGs have corresponding gene records in FlyBase already and we don't like to make duplicate records for what is actually the same gene unless we can't avoid it. The CG symbols become synonyms when an annotation is named with a more descriptive or functional name. With best regards, Rachel. ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ Rachel Drysdale. FlyBase (Cambridge), ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ Subject: Helping FlyBase: EDRC-CC13 Dear Rachel, Margit passed your message to me. You mention gene symbols that are new to FlyBase, APC4, APC7 and cdc23. Do you know which of the Genome Project CG annotations your genes correspond to? All the CGs have corresponding gene records in FlyBase already and we don't like to make duplicate records for what is actually the same gene unless we can't avoid it. The CG symbols become synonyms when an annotation is named with a more descriptive or functional name. APC4 corresponds to CG32707 (previously it was known as CG4350). We found that its sequence shows significant homology to yeast and human APC4. Based on this sequence similarity, we consider it as the Drosophila APC4 ortholog. APC7 corresponds to CG14444. It again shows similarity to human APC7, therefore it should be considered as its Drosophila ortholog. Silencing this gene by transgenic RNAi causes characteristic mitotic phenotype that doesn't affect the viability and fertility of those flies. It interacts genetically with other APC subunits. Cdc23 corresponds to CG2508. It shows sequence homology to yeast Cdc23 and to human APC8. Best wishes, Peter Deak
