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Shima, S., Aigaki, T., Nojima, T., Yamamoto, D. (2007). Identification of trf2 mutants of Drosophila with defects in anterior spiracle eversion.  Arch. Insect Biochem. Physiol. 64(4): 157--163.
FlyBase ID
FBrf0192481
Publication Type
Research paper
Abstract

TATA-box-binding protein (TBP)-related factor (Trf)2 is a member of the family of TBP-related factors present in metazoan organisms. In Drosophila, Trf2 immunoprecipitates with the nucleosome remodeling factor (NURF) chromatin remodeling complex and the DNA replication element (DRE)-binding factor DREF. When it forms a complex with DREF, Trf2 activates transcription from the DRE-binding sites of the proliferating cell nuclear antigen (PCNA) gene. Despite these observations at the molecular level, no mutations in the trf2 locus have been found in Drosophila. Here, we identify two P-element insertion alleles, PL28 and GS7403, as hypomorphic mutants with a decreased expression level of trf2. Pupae of these mutant alleles show failure in anterior spiracle eversion, a hallmark of mutations in the loci associated with ecdysteroid signaling.

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    Language of Publication
    English
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    Journal
    Abbreviation
    Arch. Insect Biochem. Physiol.
    Title
    Archives of Insect Biochemistry and Physiology
    Publication Year
    1984-
    ISBN/ISSN
    0739-4462
    Data From Reference
    Aberrations (1)
    Alleles (3)
    Genes (3)
    Natural transposons (1)
    Insertions (2)
    Transgenic Constructs (2)