The following information accompanied stocks donated to the Bloomington Stock Center by Patty Estes, University of Arizona.
P{UAS-Khc.EGFP} was generated by cloning the Khc head domain sequence (a SphI-BstI fragment corresponding to bases 248-2134 of the GenBank sequence) fused to EGFP sequence into pP{UAST}.
P{UAS-Khc.EGFP}2 is a second chromosome insertion.
P{UAS-Khc.EGFP}3 is a third chromosome insertion.
Both insertions are lethal when crossed to ubiquitous GAL4 drivers or P{GawB}elavC155, but are viable with more selective drivers.