Isolation and characterization of Df(2L)BSC187
Stacey Christensen and Kevin Cook
Bloomington Stock Center
Indiana University
Df(2L)BSC187 was isolated as a FLP recombinase-induced recombination event involving PBac{WH}CG31635f04216 and P{XP}d10866. The deletion was isolated as a chromosome lacking miniwhite markers in progeny of P{hsFLP}1, y1 w1118; PBac{WH}CG31635f04216/P{XP}d10866 males crossed to w1118; P{hs-hid}2, wgSp-1/CyO females. These males were heat shocked as larvae as described in Parks et al., Nature Genetics 36: 288-292, 2004 (FBrf0175003). This cross and crosses in preceding and succeeding generations maintained the original genetic background of the Exelixis insertion stocks (Thibault et al., Nature Genetics 36: 283-287, 2004; FBrf0175002). The recombination event generated the genetic element P+PBac{XP5.WH5}BSC187 from the segment of PBac{WH}CG31635f04216 to the left of its FRT site and the segment of P{XP}d10866 to the right of its FRT site. Its presence was verified using the PCR methods and primers described in Parks et al. Exelixis, Inc. determined the insertion site of P{XP}d10866 to be at Release 3 genomic coordinate 6701329 on chromosome arm 2L, a site predicted to be within 27A1 on both the Release 3 and Release 4 genome maps. (Note that this is not a chromosomal position predicted by the flanking sequence given in the current P{XP}d10866 insertion entry, FBti0070515.) The predicted position of PBac{WH}CG31635<up>f04216 on the Release 4 map is 26F3. Consequently, the predicted cytological breakpoints of Df(2L)BSC187 are 26F3;27A1. The presence of a deletion was confirmed cytologically, though the breakpoints were not analyzed in detail. It failed to complement cup1 and cortQW55.