Isolation and characterization of Df(3R)BSC194
Jill Gresens and Kevin Cook
Bloomington Stock Center
Indiana University
Df(3R)BSC194 was isolated as a FLP recombinase-induced recombination event involving PBac{WH}Os-Ef00970 and P{XP}Rm62d09965. The deletion was isolated as a chromosome lacking miniwhite markers in progeny of w1118; P{hs-hid}3, Dr1/TM6B, Tb1 females crossed to P{hsFLP}1, y1 w1118; PBac{WH}Os-Ef00970/P{XP}Rm62d09965 males. The males were heat shocked as larvae as described in Parks et al., Nature Genetics 36: 288-292, 2004 (FBrf0175003). This cross and crosses in preceding and succeeding generations maintained the original genetic background of the Exelixis insertion stocks (Thibault et al., Nature Genetics 36: 283-287, 2004; FBrf0175002). The recombination event generated the genetic element P+PBac{XP5.WH5}BSC194 from the segment of PBac{WH}Os-Ef00970 to the left of its FRT site and the segment of P{XP}Rm62d09965 to the right of its FRT site. Its presence was verified using the PCR methods and primers described in Parks et al. The cytological breakpoints of Df(3R)BSC194 predicted from the Release 4 genomic coordinates of the transposable element insertion sites are 83D1;83D2. The presence of a deletion was confirmed cytologically, though the breakpoints were not analyzed in detail. It failed to complement Rm62EY06795.