Isolation and characterization of Df(2L)BSC210
Stacey Christensen and Kevin Cook
Bloomington Stock Center
Indiana University
Df(2L)BSC210 was isolated as a FLP recombinase-induced recombination event involving P{XP}d02789 and PBac{WH}f07499. The deletion was isolated as a chromosome lacking miniwhite markers in progeny of P{hsFLP}1, y1 w1118; P{XP}d02789/PBac{WH}f07499 males crossed to w1118; P{hs-hid}2, wgSp-1/CyO females. These males were heat shocked as larvae as described in Parks et al., Nature Genetics 36: 288-292, 2004 (FBrf0175003). This cross and crosses in preceding and succeeding generations maintained the original genetic background of the Exelixis insertion stocks (Thibault et al., Nature Genetics 36: 283-287, 2004; FBrf0175002). The recombination event generated the genetic element P+PBac{XP5.WH5}BSC210 from the segment of P{XP}d02789 to the left of its FRT site and the segment of PBac{WH}f07499 to the right of its FRT site. Its presence was verified using the PCR methods and primers described in Parks et al. The cytological breakpoints of Df(2L)BSC210 predicted from the transposable element insertions sites using Release 4 coordinates are 31F4;32A2. It failed to complement Fatpk10307.