Isolation and characterization of Df(3R)BSC179
Stacey Christensen, Jill Gresens and Kevin Cook
Bloomington Stock Center
Indiana University
Df(3R)BSC179 was isolated as a FLP recombinase-induced recombination event involving PBac{WH}f01774 and P{XP}d10839. The deletion was isolated as a chromosome lacking miniwhite markers in progeny of w1118; P{hs-hid}3, Dr1/TM6B, Tb1 females crossed to P{hsFLP}1, y1 w1118; PBac{WH}f01774/P{XP}d10839 males. The males were heat shocked as larvae as described in Parks et al., Nature Genetics 36: 288-292, 2004 (FBrf0175003). This cross and crosses in preceding and succeeding generations maintained the original genetic background of the Exelixis insertion stocks (Thibault et al., Nature Genetics 36: 283-287, 2004; FBrf0175002). The recombination event generated the genetic element P+PBac{XP5.WH5}BSC179 from the segment of PBac{WH}f01774 to the left of its FRT site and the segment of P{XP}d10839 to the right of its FRT site. Its presence was verified using the PCR methods and primers described in Parks et al. The cytological breakpoints of Df(3R)BSC179 predicted from the Release 4 genomic coordinates of the transposable element insertions sites are 83A6;83B2.  Df(3R)BSC179 failed to complement Df(3R)Exel6144 and Itp-r83A05616.