Isolation and characterization of Df(3L)BSC284
Stacey Christensen, Jill Gresens and Kevin Cook
Bloomington Stock Center
Indiana University
Df(3L)BSC284 was isolated as a FLP recombinase-induced recombination event involving P{XP}d03105 and PBac{WH}TyrRf00961. The deletion was isolated as a chromosome lacking miniwhite markers in progeny of w1118; Dr1/TM6C, Sb1 females crossed to P{hsFLP}1, y1 w1118; P{XP}d03105/PBac{WH}TyrRf00961 males. The males were heat shocked as larvae as described in Parks et al. Nature Genetics 36: 288-292, 2004 (FBrf0175003). This cross and crosses in preceding and succeeding generations maintained the original genetic background of the Exelixis insertion stocks (Thibault et al., Nature Genetics 36: 283-287, 2004; FBrf0175002). The recombination event generated the genetic element P+PBac{XP5.WH5}BSC284 from the segment of P{XP}d03105 to the left of its FRT site and the segment of PBac{WH}TyrRf00961 to the right of its FRT site. Its presence was verified using the PCR methods and primers described in Parks et al. The cytological breakpoints of Df(3L)BSC284 predicted from the Release 5 genomic coordinates of the transposable element insertions sites are 78F1;79B1. Df(3L)BSC284 failed to complement Df(3L)Exel6137 and Mub04093.