Isolation and characterization of Df(2R)BSC334
Stacey Christensen, Kimberley Cook and Kevin Cook
Bloomington Stock Center
Indiana University
Df(2R)BSC334 was isolated as a FLP recombinase-induced recombination event involving PBac{WH}CG5765f06287 and P{XP}d06808. The deletion was isolated as a chromosome lacking miniwhite markers in progeny of P{hsFLP}1, y1 w1118; PBac{WH}CG5765f06287/P{XP}d06808 males crossed to w1118; P{hs-hid}2, wgSp-1/CyO females. These males were heat shocked as larvae as described in Parks et al., Nature Genetics 36: 288-292, 2004 (FBrf0175003). This cross and crosses in preceding and succeeding generations maintained the original genetic background of the Exelixis insertion stocks (Thibault et al., Nature Genetics 36: 283-287, 2004; FBrf0175002). The recombination event generated the genetic element P+PBac{XP5.WH5}BSC334 from the segment of PBac{WH}CG5765f06287 to the left of its FRT site and the segment of P{XP}d06808 to the right of its FRT site. Its presence was verified using the PCR methods and primers described in Parks et al. The cytological breakpoints of PBac{WH}CG5765f06287 predicted from the Release 5 genomic coordinates of the transposable element insertions sites are 55B2;55C4. It failed to complement stau1, fj1 and sbb04440. Df(2R)FDD-0378744 is a synonym for Df(2R)BSC334.