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Christensen, S., Cook, K., Cook, K. (2007.10.29). Isolation and characterization of Df(2R)BSC383. 
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Date: Mon, 29 Oct 2007  12:10:41  -0400
To: flybase-updatesXXXX, Stacey Christensen <sjchristXXXX>
From: Kevin Cook <kcook@XXXX>
Subject: Isolation and characterization of Df(2R)BSC383
Isolation and characterization of Df(2R)BSC383
Stacey Christensen, Kimberley Cook and Kevin Cook
Bloomington Stock Center
Indiana University
Df(2R)BSC383 was isolated as a FLP recombinase-induced recombination 
event involving PBac{RB}CG6347[e00490] and P{XP}mam[d01063]. The 
deletion was isolated as a chromosome lacking miniwhite markers in 
progeny of P{hsFLP}1, y[1] w[1118]; 
PBac{RB}CG6347[e00490]/P{XP}mam[d01063] males crossed to w[1118]; 
P{hs-hid}2, wg[Sp-1]/CyO females. These males were heat shocked as 
larvae as described in Parks et al., Nature Genetics 36: 288-292, 
2004 (FBrf0175003). This cross and crosses in preceding and 
succeeding generations maintained the original genetic background of 
the Exelixis insertion stocks (Thibault et al., Nature Genetics 36: 
283-287, 2004; FBrf0175002). The recombination event generated the 
genetic element P+PBac{XP5.RB3}BSC383 from the segment of 
PBac{RB}CG6347[e00490] to the left of its FRT site and the segment of 
P{XP}mam[d01063] to the right of its FRT site. Its presence was 
verified using the PCR methods and primers described in Parks et al. 
with the substitution of the primer 5'-CCAATGCGTTTATTTCAGGTCACG-3' 
for the RB3' plus or RB3' minus primer in the Hybrid PCR protocol in 
the Supplementary Methods. The cytological breakpoints of 
Df(2R)BSC383 predicted from the Release 5 genomic coordinates of the 
transposable element insertions sites are 50C6;50D2. It failed to 
complement shot[3] and mam[8]. Df(2R)FDD-0136097 is a synonym for Df(2R)BSC383.
Kevin Cook, Ph.D.               Bloomington Drosophila Stock Center
Department of Biology 
Jordan Hall 142
Indiana University              812-856-1213
1001 E. Third St.               812-855-2577 (fax)
Bloomington, IN  47405-7005     kcook@XXXX
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    Aberrations (1)
    Alleles (2)
    Genes (2)
    Insertions (3)
    Transgenic Constructs (1)