Isolation and characterization of Df(3L)BSC364
Stacey Christensen, Kimberley Cook and Kevin Cook
Bloomington Stock Center
Indiana University
Df(3L)BSC364 was isolated as a FLP recombinase-induced recombination event involving P{XP}alpha-Specd03930 and PBac{WH}zorminf00549.  The deletion was isolated as a chromosome lacking miniwhite markers in progeny of w1118; Dr1/TM6C, Sb1 females crossed to P{hsFLP}1, y1 w1118; P{XP}alpha-Specd03930/PBac{WH}zorminf00549 males. These males were heat shocked as larvae as described in Parks et al., Nature Genetics 36: 288-292, 2004 (FBrf0175003). This cross and crosses in preceding and succeeding generations maintained the original genetic background of the Exelixis insertion stocks (Thibault et al., Nature Genetics 36: 283-287, 2004; FBrf0175002). The recombination event generated the genetic element P+PBac{XP5.WH5}BSC364 from the segment of P{XP}alpha-Specd03930 to the left of its FRT site and the segment of PBac{WH}zorminf00549 to the right of its FRT site. Its presence was verified using the PCR methods and primers described in Parks et al. The cytological breakpoints of Df(3L)BSC364 predicted from the Release 5 genomic coordinates of the transposable element insertion sites are 62B4;62C4. Df(3L)BSC364 failed to complement sls1 and Df(3L)ED4284. Df(3L)FDD-0053426 is a synonym for Df(3L)BSC364.