Isolation and characterization of Df(3R)BSC503
Stacey Christensen, Kim Cook and Kevin Cook
Bloomington Stock Center
Indiana University
Df(3R)BSC503 was isolated as a FLP recombinase-induced recombination event involving P{XP}d07768 and PBac{WH}PH4alphaMPf00581. The deletion was isolated as a chromosome lacking miniwhite markers in progeny of w1118; Dr1/TM6C, Sb1 females crossed to P{hsFLP}1, y1 w1118; P{XP}d07768/PBac{WH}PH4alphaMPf00581 males. The males were heat shocked as larvae as described in Parks et al., Nature Genetics 36: 288-292, 2004 (FBrf0175003). This cross and crosses in preceding and succeeding generations maintained the original genetic background of the Exelixis insertion stocks (Thibault et al., Nature Genetics 36: 283-287, 2004; FBrf0175002). The recombination event generated the genetic element P+PBac{XP5.WH5}BSC503 from the segment of P{XP}d07768 to the left of its FRT site and the segment of PBac{WH}PH4alphaMPf00581 to the right of its FRT site. Its presence was verified using the PCR methods and primers described in Parks et al. The cytological breakpoints of Df(3R)BSC503 predicted from the Release 5 genomic coordinates of the transposable element insertions sites are 99E3;99F6. Df(3R)BSC503 failed to complement hdcFus-6, Fer1HCH00451 and Fer2LCH00035.