Isolation and characterization of Df(1)BSC544
Stacey Christensen, Kim Cook and Kevin Cook
Bloomington Stock Center
Indiana University
Df(1)BSC544 was isolated as a FLP recombinase-induced recombination event involving PBac{WH}f04060 and P{XP}Tis11d04268. The deletion was isolated as a chromosome lacking miniwhite markers in progeny of PBac{WH}f04060/P{XP}Tis11d04268; MKRS, P{hsFLP}86E/+ females crossed to Binsinscy/Y males. These females were heat shocked as larvae as described in Parks et al., Nature Genetics 36: 288-292, 2004 (FBrf0175003). This cross and crosses in preceding and succeeding generations maintained the original genetic background of the Exelixis insertion stocks (Thibault et al., Nature Genetics 36: 283-287, 2004; FBrf0175002). The recombination event generated the genetic element P+PBac{XP5.WH5}BSC544 from the segment of PBac{WH}f04060 to the left of its FRT site and the segment of P{XP}Tis11d04268 to the right of its FRT site. Its presence was verified using the PCR methods and primers described in Parks et al. The cytological breakpoints of Df(1)BSC544 predicted from the Release 5 genomic coordinates of the transposable element insertions sites are 10E4;11B9. It failed to complement gd7 and fw1.