The following information accompanied stocks donated to the Bloomington Stock Center by Jack Bateman, Harvard Medical School.
The P{attP.w+.attP} construct may be used for Recombination-Mediated Cassette Exchange.  The mini-white gene is flanked by inverted attP sites. P{attP.w+.attP} is a derivative of P{UASTP2} (FBtp0021963) in which the UAS and TATA sequences adjacent to the attP-miniwhite-attP cassette have been removed. Specifically, P{UASTP2} was digested with BamHI and StuI to remove UAS-TATA-attP, and an attP-containing BamHI-EcoRV fragment from pTA-attP (described in Groth et al., 2000, Proc. Natl. Acad. Sci. USA 97: 5995-6000) was inserted.
The sequence of P{attP.w+.attP} is given by Genbank accession EU761203.
The following insertions are homozygous viable and fertile:
P{attP.w+.attP}JB37B
sequence from: 5' end
position:  2L:18964658  (Release 4.3)
Predicted cytology:  37B7
strand: bottom
nearest gene: 8 bp upstream of Nak
P{attP.w+.attP}JB38F
sequence from: 3' end
position:  2L:20810313  (Release 4.3)
Predicted cytology:  38F1
strand: top
nearest gene: 1.7 kb upstream of CG9331
P{attP.w+.attP}JB53F
sequence from: 3' end
position:  2R:12612373  (Release 4.3)
Predicted cytology:  53F8
strand: top
nearest gene: 80 bp upstream of GstS1
P{attP.w+.attP}TaraJB89B
sequence from: 3' end
position:  3R:12051747  (Release 4.3)
Predicted cytology:  89B9
strand: bottom
nearest gene: 376 bp into 5' UTR of Tara