Isolation and characterization of Df(2R)BSC602
Stacey Christensen, Kim Cook and Kevin Cook
Bloomington Stock Center
Indiana University
Df(2R)BSC602 was isolated as a FLP recombinase-induced recombination event involving PBac{WH}CG15874f03854 and P{XP}d09580. The deletion was isolated as a chromosome lacking miniwhite markers in progeny of P{hsFLP}1, y1 w1118; PBac{WH}CG15874f03854/P{XP}d09580 males crossed to w1118; P{hs-hid}2, wgSp-1/SM6a females. These males were heat shocked as larvae as described in Parks et al., Nature Genetics 36: 288-292, 2004 (FBrf0175003). This cross and crosses in preceding and succeeding generations maintained the original genetic background of the Exelixis insertion stocks (Thibault et al., Nature Genetics 36: 283-287, 2004; FBrf0175002). The recombination event generated the genetic element P+PBac{XP5.WH5}BSC602 from the segment of PBac{WH}CG15874f03854 to the left of its FRT site and the segment of P{XP}d09580 to the right of its FRT site. Its presence was verified using the PCR methods and primers described in Parks et al. The cytological breakpoints of Df(2R)BSC602 predicted from the Release 5 genomic coordinates of the transposable element insertions sites are 60C8;60E5. Df(2R)BSC602 failed to complement pio2R-16, Dll5 and Eps-15EP2513.