Isolation and characterization of Df(2R)BSC606
Stacey Christensen, Kim Cook and Kevin Cook
Bloomington Stock Center
Indiana University
Df(2R)BSC606 was isolated as a FLP recombinase-induced recombination event involving P{XP}CG13594d00307 and PBac{WH}f05641. The deletion was isolated as a chromosome lacking miniwhite markers in progeny of P{hsFLP}1, y1 w1118; P{XP}CG13594d00307/PBac{WH}f05641 males crossed to w1118; P{hs-hid}2, wgSp-1/SM6a females. These males were heat shocked as larvae as described in Parks et al., Nature Genetics 36: 288-292, 2004 (FBrf0175003). This cross and crosses in preceding and succeeding generations maintained the original genetic background of the Exelixis insertion stocks (Thibault et al., Nature Genetics 36: 283-287, 2004; FBrf0175002). The recombination event generated the genetic element P+PBac{XP5.WH5}BSC606 from the segment of P{XP}CG13594d00307 to the left of its FRT site and the segment of PBac{WH}f05641 to the right of its FRT site. Its presence was verified using the PCR methods and primers described in Parks et al. Exelixis, Inc. determined the insertion site of P{XP}CG13594d00307 to be at Release 3 genomic coordinate 19674600 on chromosome arm 2R This corresponds to 60D10 on the Release 3 and Release 5 genome maps. The predicted position of PBac{WH}f05641 on the Release 5 map is 60E1. Consequently, the cytological breakpoints of Df(2R)BSC606 are predicted to be 60D10;60E1. Df(2R)BSC606 failed to complement Mmp1k04809 and Eps-15EP2513.