Isolation and characterization of Df(2R)BSC594
Stacey Christensen, Kim Cook and Kevin Cook
Bloomington Stock Center
Indiana University
Df(2R)BSC594 was isolated as a FLP recombinase-induced recombination event involving P{XP}d08264 and PBac{WH}f06710. The deletion was isolated as a chromosome lacking miniwhite markers in progeny of P{hsFLP}1, y1 w1118; P{XP}d08264/PBac{WH}f06710 males crossed to w1118; P{hs-hid}2, wgSp-1/CyO females. These males were heat shocked as larvae as described in Parks et al., Nature Genetics 36: 288-292, 2004 (FBrf0175003). This cross and crosses in preceding and succeeding generations maintained the original genetic background of the Exelixis insertion stocks (Thibault et al., Nature Genetics 36: 283-287, 2004; FBrf0175002). The recombination event generated the genetic element P+PBac{XP5.WH5}BSC594 from the segment of P{XP}d08264 to the left of its FRT site and the segment of PBac{WH}f06710 to the right of its FRT site. Its presence was verified using the PCR methods and primers described in Parks et al. Exelixis, Inc. determined the insertion site of P{XP}d08264 to be Release 3 genomic coordinate 14695698 on chromosome arm 2R. The Gene Disruption project determined the insertion site of P{XP}d08264 to be Release 3 genomic coordinate 14695744 on arm 2R. This corresponds to 56E1 on the Release 3 and Release 5 genome maps. The predicted position of PBac{WH}f06710 on the Release 5 map is 56F9. Consequently, the cytological breakpoints of Df(2R)BSC594 are predicted to be 56E1;56F9. Df(2R)BSC594 failed to complement Df(2R)BSC22, 18wDelta7-35, hrg1 and hrg10.