Isolation and characterization of Df(2R)BSC630
Stacey Christensen, Kim Cook and Kevin Cook
Bloomington Stock Center
Indiana University
Df(2R)BSC630 was isolated as a FLP recombinase-induced recombination event involving PBac{WH}gusf04367 and P{XP}CG11163d00154. The deletion was isolated as a chromosome lacking miniwhite markers in progeny of P{hsFLP}1, y1 w1118; PBac{WH}gusf04367/P{XP}CG11163d00154 males crossed to w1118; P{hs-hid}2, wgSp-1/CyO females. These males were heat shocked as larvae as described in Parks et al., Nature Genetics 36: 288-292, 2004 (FBrf0175003). This cross and crosses in preceding and succeeding generations maintained the original genetic background of the Exelixis insertion stocks (Thibault et al., Nature Genetics 36: 283-287, 2004; FBrf0175002). The recombination event generated the genetic element P+PBac{XP5.WH5}BSC630 from the segment of PBac{WH}gusf04367 to the left of its FRT site and the segment of P{XP}CG11163d00154 to the right of its FRT site. Its presence was verified using the PCR methods and primers described in Parks et al. Exelixis, Inc. determined the insertion site of PBac{WH}gusf04367 to be Release 3 genomic coordinate 266687 on 2R. This corresponds to 41D3 on the Release 5 genome map. The predicted position of P{XP}CG11163d00154 on the Release 5 map is 41F11. Consequently, the cytological breakpoints of Df(2R)BSC630 are predicted to be 41D3;41F11. Df(2R)BSC630 failed to complement l(2)NC136KG10496 and ap54.