FlyBase Allele Report: Dmel\Not3[KG10496]

General Information

Symbol

Dmel\Not3^KG10496

Species

D. melanogaster

Name

FlyBase ID

FBal0148147

Feature type

allele

Associated gene

Dmel\Not3

Associated Insertion(s)

P{SUPor-P}Not3^KG10496

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

P-element activity

Nature of the Allele

Allele class

Mutagen

P-element activity

(Myster and Peifer, 2003.9.11, Gene Disruption Project members, 2001-)

Progenitor genotype

Associated Insertion(s)

P{SUPor-P}Not3^KG10496

Cytology

Description

Allele components

Component

Use(s)

Inserted element

P{SUPor-P}

insertional mutagenesis tool

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

transposable_element_insertion_site

2R:5,662,767..5,662,767 [-]

(Vicente et al., 2018, De Keersmaecker et al., 2013, Myster et al., 2004, Myster and Peifer, 2003.9.11, Gene Disruption Project members, 2001-)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

exacerbates cancer

modeled by Delta^UAS.cDa

(De Keersmaecker et al., 2013)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

lethal

(Neely et al., 2010)

lethal | recessive

(Myster and Peifer, 2003.9.11)

Phenotype Manifest In

larval pericardial cell

(Neely et al., 2010)

Detailed Description

Statement

Reference

Not3^KG10496 heterozygosity does not induce visible eye defects; the eye disc exhibits no changes in cell division (BrdU labelling and pH3 immunostaining), or apoptosis (Dcp1a immunostaining), as compared to controls.

(Vicente et al., 2018)

The pericardial cells are misarranged in mutant stage 17 embryos, while the inner cardioblast cell row is normal.

(Neely et al., 2010)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

Not3[+]/Not3^KG10496 is an enhancer of increased occurrence of cell division | larval stage phenotype of Delta^UAS.cDa, Scer\GAL4^ey.PH

(Vicente et al., 2018)

Not3[+]/Not3^KG10496 is an enhancer of visible | adult stage phenotype of Delta^UAS.cDa, Scer\GAL4^ey.PH

(Vicente et al., 2018)

Not3[+]/Not3^KG10496 is an enhancer of neoplasia | adult stage phenotype of Delta^UAS.cDa, Scer\GAL4^ey.PH

(Vicente et al., 2018)

NOT Enhancer of

Statement

Reference

Not3^KG10496/Not3^KG10496 is a non-enhancer of neoplasia | adult stage | male phenotype of tut⁴

(Shan et al., 2017)

Other

Statement

Reference

Delta^UAS.cDa, Not3^KG10496, Scer\GAL4^ey.PH has neoplasia phenotype

(De Keersmaecker et al., 2013)

Phenotype Manifest In

Enhancer of

Statement

Reference

Not3[+]/Not3^KG10496 is an enhancer of eye phenotype of Delta^UAS.cDa, Scer\GAL4^ey.PH

(Vicente et al., 2018)

Not3[+]/Not3^KG10496 is an enhancer of eye disc phenotype of Delta^UAS.cDa, Scer\GAL4^ey.PH

(Vicente et al., 2018)

Not3^KG10496 is an enhancer of eye phenotype of Delta^UAS.cDa, Scer\GAL4^ey.PH

(De Keersmaecker et al., 2013)

NOT Enhancer of

Statement

Reference

Not3^KG10496/Not3^KG10496 is a non-enhancer of testis phenotype of tut⁴

(Shan et al., 2017)

Not3^KG10496/Not3^KG10496 is a non-enhancer of spermatogonium phenotype of tut⁴

(Shan et al., 2017)

Additional Comments

Genetic Interactions

Statement

Reference

The severity of the tut⁴ mutant tumorigenic phenotype is also strongly enhanced by combination with Not3^KG10496 homozygosity, on the contrary the phenotype appears rather ameliorated. If combined with just one copy of tut⁴, this genetic perturbation does not cause a significant tumor phenotype.

(Shan et al., 2017)

Addition of Not3^KG10496 to the Scer\GAL4^ey.PH Dl^Scer\UAS.cDa background markedly increases tumor incidence in the eyes.

(De Keersmaecker et al., 2013)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Fails to complement

Not3^NC136

(Myster and Peifer, 2003.9.11)

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

15271

y¹; P{SUPor-P}Not3^KG10496/CyO; ry⁵⁰⁶

Notes on Origin

Discoverer

Comments

Excision of the insertion completely reverts the mutant embryonic heart phenotype.

(Neely et al., 2010)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

CG8426^KG10496

(Neely et al., 2010)

Not3^KG10496

(Shan et al., 2017)

l(2)NC136^KG10496

(Gambetta and Müller, 2009.8.18, Christensen et al., 2008.9.29, Christensen et al., 2008.12.28)

Name Synonyms

Secondary FlyBase IDs

References (10)

Report Sections

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