Isolation and characterization of Df(1)BSC657
Stacey Christensen, Kim Cook and Kevin Cook
Bloomington Stock Center
Indiana University
Df(1)BSC657 was isolated as a FLP recombinase-induced recombination event involving P{XP}d05473 and PBac{RB}pone02353. The deletion was isolated as a chromosome lacking miniwhite markers in progeny of w1118 P{XP}d05473/w1118 PBac{RB}pone02353; MKRS, P{hsFLP}86E/+ females crossed to Binsinscy/Y males. These females were heat shocked as larvae as described in Parks et al., Nature Genetics 36: 288-292, 2004 (FBrf0175003). This cross and crosses in preceding and succeeding generations maintained the original genetic background of the Exelixis insertion stocks (Thibault et al., Nature Genetics 36: 283-287, 2004; FBrf0175002). The recombination event generated the genetic element P+PBac{XP5.RB3}BSC657 from the segment of P{XP}d05473 to the left of its FRT site and the segment of PBac{RB}pone02353 to the right of its FRT site. Its presence was verified using the PCR methods and primers described in Parks et al. with the substitution of the primer 5'-GCTTCTAAACGCTTACGCATAAACGATG-3' for the RB3' plus or RB3' minus primer in the Hybrid PCR protocol in the Supplementary Methods. The cytological breakpoints of Df(1)BSC657 predicted from the Release 5 genomic coordinates of the transposable element insertions sites are 4B6;4C10. It failed to complement bi1, rb1 and peb1.