Isolation and characterization of Df(2L)BSC694
Stacey Christensen, Kim Cook and Kevin Cook
Bloomington Stock Center
Indiana University
Df(2L)BSC694 was isolated as a FLP recombinase-induced recombination event involving PBac{WH}mRpS23f07522 and P{XP}d03952. The deletion was isolated as a chromosome carrying two copies of the miniwhite marker in progeny of P{hsFLP}1, y1 w1118; PBac{WH}mRpS23f07522/P{XP}d03952 males crossed to w1118; P{hs-hid}2, wgSp-1/SM6a females. These males were heat shocked as larvae as described in Parks et al., Nature Genetics 36: 288-292, 2004 (FBrf0175003). This cross and crosses in preceding and succeeding generations maintained the original genetic background of the Exelixis insertion stocks (Thibault et al., Nature Genetics 36: 283-287, 2004; FBrf0175002). The recombination event generated the genetic element P+PBac{XP3.WH3}BSC694 from the segment of PBac{WH}mRpS23f07522 to the left of its FRT site and the segment of P{XP}d03952 to the right of its FRT site. Exelixis, Inc. determined the insertion site of P{XP}d03952 to be Release 3 genomic coordinate 13995884 on chromosome arm 2L. This corresponds to 34F1 on the Release 3 and Release 5 genome maps. The predicted position of PBac{WH}mRpS23f07522 on the Release 5 map is 34D6. Consequently, the cytological breakpoints of Df(2L)BSC694 are predicted to be 34D6;34F1. Df(2L)BSC694 failed to complement Df(2L)BSC345 for viability and Ance34Eb-2 for male fertility.