FB2026_03 , released September 17, 2026
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Parks, A., Muskavitch, M. (2009.3.2). P{UAS-Chc.DN} construct and insertions. 
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FBrf0207416
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Personal communication to FlyBase
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The following information accompanied stocks donated to the Bloomington Stock Center by Annette Parks and Marc Muskavitch, Boston College.
P{UAS-Chc.DN} expresses a dominant negative Chc protein under UAS control consisting of carboxy-terminal amino acids 1075 to 1678. BglII and KpnI sites and a start codon were introduced to flank the coding sequence via PCR amplification using the following oligonucleotides (Chc sequences are indicated in uppercase): 5'-ggagatctcaac atgAAGTTCGATGTGAAC-3' (5' oligo) and 5'-gcggtaccTCACAAGTAGGGATAGCCCAT-3' (3' oligo). The amplified fragment was subcloned into the BglII and KpnI sites of pP{UAST}.
P{UAS-Chc.DN}2 is a second chromosome insertion.
P{UAS-Chc.DN}3 is a third chromosome insertion.
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    Language of Publication
    English
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    Alleles (2)
    Genes (2)
    Natural transposons (1)
    Insertions (2)
    Experimental Tools (1)
    Transgenic Constructs (1)