Isolation and characterization of Df(3L)BSC734
Stacey Christensen, Kim Cook and Kevin Cook
Bloomington Stock Center
Indiana University
Df(3L)BSC734 was isolated as a FLP recombinase-induced recombination event involving PBac{WH}f04670 and P{XP}trbld03196. The deletion was isolated as a chromosome lacking miniwhite markers in progeny of w1118; P{hs-hid}3, Dr1/TM6C, Sb1 cu1 females crossed to P{hsFLP}1, y1 w1118; PBac{WH}f04670/P{XP}trbld03196 males. The males were heat shocked as larvae as described in Parks et al., Nature Genetics 36: 288-292, 2004 (FBrf0175003). This cross and crosses in preceding and succeeding generations maintained the original genetic background of the Exelixis insertion stocks (Thibault et al., Nature Genetics 36: 283-287, 2004; FBrf0175002). The recombination event generated the genetic element P+PBac{XP5.WH5}BSC734 from the segment of PBac{WH}f04670 to the left of its FRT site and the segment of P{XP}trbld03196 to the right of its FRT site. Its presence was verified using the PCR methods and primers described in Parks et al. The cytological breakpoints of Df(3L)BSC734 predicted from the Release 5 genomic coordinates of the transposable element insertion sites are 77A4;77C1. Df(3L)BSC734 failed to complement polo16-1 and fbl1.