Isolation and characterization of Df(3L)BSC773
Stacey Christensen, Kim Cook and Kevin Cook
Bloomington Stock Center
Indiana University
Df(3L)BSC773 was isolated as a FLP recombinase-induced recombination event involving P{XP}d09477 and PBac{WH}fryf01845. The deletion was isolated as a chromosome carrying two copies of the miniwhite marker in progeny of w1118; P{hs-hid}3, Dr1/TM6C, Sb1 cu1 females crossed to P{hsFLP}1, y1 w1118; P{XP}d09477/PBac{WH}fryf01845 males. The males were heat shocked as larvae as described in Parks et al., Nature Genetics 36: 288-292, 2004 (FBrf0175003). This cross and crosses in preceding and succeeding generations maintained the original genetic background of the Exelixis insertion stocks (Thibault et al., Nature Genetics 36: 283-287, 2004; FBrf0175002). The recombination event generated the genetic element P+PBac{XP3.WH3}BSC773 from the segment of P{XP}d09477to the left of its FRT site and the segment of PBac{WH}fryf01845 to the right of its FRT site. Exelixis, Inc. determined the insertion site of P{XP}d09477 to be Release 3 genomic coordinate 9423408 on chromosome arm 3L, which corresponds to Release 5 coordinate  3L:9458979 . The insertion site of PBac{WH}fryf01845 is Release 5 genomic coordinate  3L:9671802 . Consequently, the molecular breakpoints of Df(3L)BSC773 predicted from the Release 5 genomic coordinates of the transposable element insertion sites are  3L:9458979 ;9671802 and the cytological breakpoints predicted from these coordinates are 67B8;67C4. Df(3L)BSC773 failed to complement Uch-L3j2B8 and pathKG06640.