Subject: P{UAS-Psn.541.M255V}
The following information was provided to the Bloomington Stock Center by Annette Parks, Boston College.
The description of P{UAS-Psn.541.M157V} provided in /reports/FBrf0178861.html was incorrect. It stated "In P{UAS-Psn.541.M157V}, Psn sequences encoding a M157V variant of the 541 amino acid isoform were cloned into P{Express-UAS}. The M157V change in flies mimics the M239V change in humans." In fact, the construct encodes a M255V variant of the 541 amino acid isoform. This is the change that actually mimics the M239V change in humans.
The name of the construct should be changed to P{UAS-Psn.541.M255V} to avoid confusion. This change affects entry http://flybase.net/reports/FBtp0018532.html and the entries for three associated insertions:
P{UAS-Psn.541.M157V}1 http://flybase.net/reports/FBti0040718.html
P{UAS-Psn.541.M157V}2 http://flybase.net/reports/FBti0040719.html
P{UAS-Psn.541.M157V}3 http://flybase.net/reports/FBti0040720.html
In addition, the http://flybase.net/reports/FBtp0018540.html entry for P{UAS-Psn.527.D447A} needs a bit of clarification. The report /reports/FBrf0178861.html says "The D447A change in flies mimics the D385A change in humans." This implies that there is a naturally occurring D385A mutation in humans. In fact, this protein variant was produced in vitro to study the function of human Psn. It served as the basis for designing the fly D447A variant.