Isolation and characterization of Df(3R)BSC847
Stacey Christensen, Kim Cook and Kevin Cook
Bloomington Stock Center
Indiana University
Df(3R)BSC847 was isolated as a FLP recombinase-induced recombination event involving P{XP}GstD3d06796 and PBac{WH}f00021. The deletion was isolated as a chromosome lacking miniwhite markers in progeny of w1118; P{hs-hid}3, Dr1/TM6C, Sb1 cu1 females crossed to P{hsFLP}1, y1 w1118; P{XP}GstD3d06796/PBac{WH}f00021 males. The males were heat shocked as larvae as described in Parks et al., Nature Genetics 36: 288-292, 2004 (FBrf0175003). This cross and crosses in preceding and succeeding generations maintained the original genetic background of the Exelixis insertion stocks (Thibault et al., Nature Genetics 36: 283-287, 2004; FBrf0175002). The recombination event generated the genetic element P+PBac{XP5.WH5}BSC847 from the segment of P{XP}GstD3d06796 to the left of its FRT site and the segment of PBac{WH}f00021 to the right of its FRT site. Its presence was verified using the PCR methods and primers described in Parks et al. The breakpoints of Df(3R)BSC847 predicted from the Release 5 genomic coordinates of the transposable element insertion sites are  3R:8198586 ;8388182 and the cytological breakpoints predicted from these coordinates are 87B8;87C1. Df(3R)BSC847 failed to complement Pp1-87b1.