FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
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Cook, K., Christensen, S., Cook, K. (2009.11.25). Isolation and characterization of Df(1)BSC851. 
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FBrf0209447
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Personal communication to FlyBase
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Df(1)BSC851 was isolated as a FLP recombinase-induced recombination event involving P{XP}d06616 and PBac{WH}f03966. The deletion was isolated as a chromosome lacking miniwhite markers in progeny of P{XP}d06616/PBac{WH}f03966; MKRS, P{hsFLP}86E/+ females crossed to Binsinscy/Y males. These females were heat shocked as larvae as described in Parks et al., Nature Genetics 36: 288-292, 2004 (FBrf0175003). This cross and crosses in preceding and succeeding generations maintained the original genetic background of the Exelixis insertion stocks (Thibault et al., Nature Genetics 36: 283-287, 2004; FBrf0175002). The recombination event generated the genetic element P+PBac{XP5.WH5}BSC851 from the segment of P{XP}d06616 to the left of its FRT site and the segment of PBac{WH}f03966 to the right of its FRT site. Its presence was verified using the PCR methods and primers described in Parks et al. The breakpoints of Df(1)BSC851 predicted from the Release 5 genomic coordinates of the transposable element insertion sites are  X:12477249 ;12790640--12790767 and the cytological breakpoints predicted from these coordinates are 11B2;11D1. It failed to complement l(1)G0060G0060 and Df(1)G0124.
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    English
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    Aberrations (2)
    Alleles (1)
    Genes (1)
    Insertions (3)
    Transgenic Constructs (1)