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Citation
Egelhofer, T.A., Minoda, A., Klugman, S., Lee, K., Kolasinska-Zwierz, P., Alekseyenko, A.A., Cheung, M.S., Day, D.S., Gadel, S., Gorchakov, A.A., Gu, T., Kharchenko, P.V., Kuan, S., Latorre, I., Linder-Basso, D., Luu, Y., Ngo, Q., Perry, M., Rechtsteiner, A., Riddle, N.C., Schwartz, Y.B., Shanower, G.A., Vielle, A., Ahringer, J., Elgin, S.C., Kuroda, M.I., Pirrotta, V., Ren, B., Strome, S., Park, P.J., Karpen, G.H., Hawkins, R.D., Lieb, J.D. (2011). An assessment of histone-modification antibody quality.  Nat. Struct. Mol. Biol. 18(1): 91--93.
FlyBase ID
FBrf0212707
Publication Type
Research paper
Abstract

We have tested the specificity and utility of more than 200 antibodies raised against 57 different histone modifications in Drosophila melanogaster, Caenorhabditis elegans and human cells. Although most antibodies performed well, more than 25% failed specificity tests by dot blot or western blot. Among specific antibodies, more than 20% failed in chromatin immunoprecipitation experiments. We advise rigorous testing of histone-modification antibodies before use, and we provide a website for posting new test results (http://compbio.med.harvard.edu/antibodies/).

PubMed ID
PubMed Central ID
PMC3017233 (PMC) (EuropePMC)
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Secondary IDs
    Language of Publication
    English
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    Journal
    Abbreviation
    Nat. Struct. Mol. Biol.
    Title
    Nature Structural and Molecular Biology
    Publication Year
    2004-
    ISBN/ISSN
    1545-9993 1545-9985
    Data From Reference
    Genes (6)