FB2026_02 , released June 18, 2026
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Transgenic RNAi Project, (2011.6.14). Control RNAi constructs and insertions from the Transgenic RNAi Project. 
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FBrf0213920
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Personal communication to FlyBase
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The following information accompanied stocks donated to the Bloomington Drosophila Stock Center by the Transgenic RNAi Project (TRiP - http://www.flyrnai.org/TRiP-HOME.html).
P{VALIUM20-EGFP} consists of a short hairpin RNA for RNAi of EGFP and various EGFP derivatives (ECFP, EYFP and YFP-2, but not plain GFP) under the control of UAS in the VALIUM20 vector (FBmc0003034). The hairpin was made using the following oligos - forward: ctagcagtCAAGGACGACGGCAACTACAAtagttatattcaagcataTTGTAGTTGCCGTCGTCCTTGgcg, reverse: aattcgcCAAGGACGACGGCAACTACAAtatgcttgaatataactaTTGTAGTTGCCGTCGTCCTTGactg.
P{VALIUM20-EGFP}attP2 is a homozygous viable and fertile insertion into the attP2 site (68A4,  3L:11063638 ) on the third chromosome.
P{VALIUM20-GAL4.1} consists of a short hairpin RNA for RNAi of GAL4 under the control of UAS in the VALIUM20 vector (FBmc0003034). The hairpin was made using the following oligos -  forward:ctagcagtCAGGATTATTTGTACAAGATAtagttatattcaagcataTATCTTGTACAAATAATCCTGgcg ,  reverse:aattcgcCAGGATTATTTGTACAAGATAtatgcttgaatataactaTATCTTGTACAAATAATCCTGactg .
P{VALIUM20-GAL4.1}attP2 is a homozygous viable and fertile insertion into the attP2 site (68A4,  3L:11063638 ) on the third chromosome.
P{VALIUM20-GAL4.2} consists of a short hairpin RNA for RNAi of GAL4 under the control of UAS in the VALIUM20 vector (FBmc0003034). The hairpin was made using the following oligos -  forward:ctagcagtTCGGAAGAGAGTAGTAACAAAtagttatattcaagcataTTTGTTACTACTCTCTTCCGAgcg ,  reverse:aattcgcTCGGAAGAGAGTAGTAACAAAtatgcttgaatataactaTTTGTTACTACTCTCTTCCGAactg .
P{VALIUM20-GAL4.2}attP2 is a homozygous viable and fertile insertion into the attP2 site (68A4,  3L:11063638 ) on the third chromosome.
P{VALIUM20-mCherry} consists of a short hairpin RNA for RNAi of the mCherry form of RFP under the control of UAS in the VALIUM20 vector (FBmc0003034). The hairpin was made using the following oligos -  forward:ctagcagtCGAGTTCATCTACAAGGTGAAtagttatattcaagcataTTCACCTTGTAGATGAACTCGgcg , reverse: aattcgcCGAGTTCATCTACAAGGTGAAtatgcttgaatataactaTTCACCTTGTAGATGAACTCGactg.
P{VALIUM20-mCherry}attP2 is a homozygous viable and fertile insertion into the attP2 site (68A4,  3L:11063638 ) on the third chromosome.
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    English
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    Alleles (4)
    Genes (3)
    Molecular Constructs (1)
    Natural transposons (1)
    Insertions (4)
    Experimental Tools (2)
    Transgenic Constructs (4)