FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Recombinant Construct: pVALIUM20
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General Information
Symbol
pVALIUM20
FlyBase ID
FBmc0003034
Feature type
engineered_plasmid
Component Allele(s)
Size
8.257
Expression Data
Associated insertion(s)
0 available
Molecular map
Description and Uses
Description

pVALIUM20 allows cloning of short hairpins as annealed synthetic oligos, targeted against specific genes via the production of short hairpin microRNAs; contains v+t1.8 as a selectable marker; the hsp70 basal promoter; a multiple cloning site (MCS) for cloning shRNAs in a mir-1 scaffold; a ftz 3'UTR intron followed by a SV40 3'UTR to facilitate shRNA processing and export from the nucleus; two pentamers of UAS, one of which is flanked by loxP sites and can thus be excised using the P1cre recombinase to generate a 5xUAS derivative; two gypsy insulator sequences flanking the transcription unit to enhance hairpin DNA transcription; an attB sequence to allow phiC31:int-targeted integration at genomic attP landing sites.

(Ni et al., 2010.12.1)
Construct components
Component
Use(s)
Regulatory region(s)
UAS
Also carries
loxP
Marker(s)
v+t1.8
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA Sequence
Extent
Segments and Size
Total Size

8.257

(Transgenic RNAi Project, 2017)
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Phenotypic Data
Component Allele
Phenotypic Data
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Progenitors and Descendants
Comments

Efficacy of pVALIUM20-based vs pVALIUM22-based RNAi constructs in germline and soma compared. pVALIUM20 generates effective knockdown phenotypes in both germline and soma; pVALIUM22 is more effective for knockdowns in the germline and effects appear to be restricted to the female germline (excluding somatic ovarian cells), but is significantly less effective in the soma.

(Ni et al., 2011)

See TRiP(http://fgr.hms.harvard.edu/trip-plasmid-vector-sets) for downloadable maps, sequences and cloning protocols.

(Transgenic RNAi Project, 2017)

'VALIUM' stands for Vermilion-AttB-Loxp-Intron-UAS-MCS. pVALIUM20 triggers effective RNAi knockdown and corresponding phenotypes in somatic tissue (stronger than pVALIUM10) and also works well in germline (not as strong as pVALIUM22).

(Ni et al., 2010.12.1)
Stocks (0)
Stocks containing insertion(s) of this recombinant construct
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
Valium20
(Ni et al., 2011)
pVALIUM20
(FlyBase, 1996-)
Secondary FlyBase IDs
    References (6)