FB2026_03 , released September 17, 2026
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Gonsalvez, G. (2017.2.21). Tm1 deletion and source of S2 cells. 
FlyBase ID
FBrf0234860
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Personal communication to FlyBase
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Text of Personal Communication 
Regarding the Tm1delC deletion, this is the sequence on the 5’side of the break point:
AACAGAGGCAGCAGAAACAATTGTTCAAAAGTGATAAGG (break)
This is the sequence on the 3’side of the break point:
(break)ATGAGGGGGCCAGTAATCCGGCAGCAGCTTTAATAG
This is the sequence that is missing in the null:
CGAAGGAGAAGTCCAGCAGGCAGCAGAGAAAGCGAGCCGGCACCAGGAGCACTCGCAATCCGAGGACGCAATCGGAATCTTCGGGCCTGTCATCCCGTTGGTGTTCCATCGAAGAGCAGTTGAACCTGCTGGACGACCTGCTCTACTACTGCGATGAGGAGGCCGAACTGTATCTAGCCCAGCTGTACGAAAGGTTCGAGACACTCCGGCGTAGCAGGAGTTCCTCGCCAGTGAGCGATTTCTGGAGCGATTCCGACATGGAGCACGATGACTCCAGCACCAGTGGCACTGGCAGCCATACAGCCAGCAATACCAGTCTAGTTCCCGCCTCGCTGAAGCGTCGTGGCCACCAGCATCATCCGCGATTCTCGGGCACTCGCCGTCCCAATGTGCCCAATGTCCAGGAGATTCTGGCCGCACTCTATCGTGGCGACTCAAAGAGCGCCCTGTCCAATCTGAGAGGAGAGACGCAACCGGAGGAGGAGCAACCACAGCAGCAGACGGAGGAGGTGCTGCCACCCAGCAGAAGCACTTTAAGCCTGCCCCTCAGTGAATCGGTGACCAATTCCCTGGGCAGCAACAGTCCCACGCCCACAGACGAAAGCAGCGTGCAAG
The cell line used was S2 (not D-mel2) from Invitrogen/Life technologies.
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