The genomic constructs for Tret1-1 and Treh are inserted at landing site 86Fb and the construct for Pyk is inserted in 44F. The inserted Plasmid includes a mini white and the genomic regions of the respective genes with an added 3x HA-tag at the C-terminus (sequence: ATGGATCTCCACCGCGGTGGAGGCCGCATCTTTTACCCATACGATGTTCCTGACTATGCGGGCTATCCCTATGACGTCCCGGACTATGCAGGATCCTATCCATATGACGTTCCAGATTACGCTGCTCATGGCGGA) . The genomic regions cloned are: Tret1-1 : 11,660,494 to 11,677,935. Treh : 21,072,790 to 21,088,385. Pyk : 22,367,155 to 22,373,236. The inserted sequence in the CG6262I-2 allele is TTGATCATGACTC.