TpnC41C deletion (TpnC41Cδ2)
CRISPR targets:
5’end:   5’-gctattcagctagtgagagc-3’
3’end:   5’-gtattttcgactgcttagat-3’
Primers used for sequencing of the PCR fragment amplified from genomic DNA of the TpnC41Cδ2
Forward:  (TpnC41C-838F)   5’-gtctcctctagctcttgg-3’
Reverse:  (TpnC41C+234R)   5’-caataggctgatacgcac-3’
Deletion coordinates:
 2R:5070863 …5065383
Double knockout TpnC41C (TpnC41Cδ2) and TpnC4 (TpnC4δ6) 
CRISPR targets:
5’end:   5’-ggccgatggagaatacgaca-3’
3’end:   5’-gaattcatgcaagttatgacagg-3’
Primers used for sequencing of the PCR fragment amplified from genomic DNA of the TpnC4δ6
Forward:  5’-aaatacaacatacccgtccacc-3’
Reverse:  5’-ccacccacgtgcaaaaaa-3’
Insertion coordinates:
Insertion of “T” between  2R:5506706  and  2R:5506707  resulting in frameshift and producing a stop codon TAG after Leu-14;
 2R:5506693   insertion of “T”