Arii, J., Watanabe, M., Maeda, F., Tokai-Nishizumi, N., Chihara, T., Miura, M., Maruzuru, Y., Koyanagi, N., Kato, A., Kawaguchi, Y. (2018). ESCRT-III mediates budding across the inner nuclear membrane and regulates its integrity.  Nat. Commun. 9(1): 3379.

FBrf0241219

Research paper

Vesicle-mediated nucleocytoplasmic transport is a nuclear pore-independent mechanism for the nuclear export of macromolecular complexes, but the molecular basis for this transport remains largely unknown. Here we show that endosomal sorting complex required for transport-III (ESCRT-III) is recruited to the inner nuclear membrane (INM) during the nuclear export of herpes simplex virus 1 (HSV-1). Scission during HSV-1 budding through the INM is prevented by depletion of ESCRT-III proteins. Interestingly, in uninfected human cells, the depletion of ESCRT-III proteins induces aberrant INM proliferation. Our results show that HSV-1 expropriates the ESCRT-III machinery in infected cells for scission of the INM to produce vesicles containing progeny virus nucleocapsids. In uninfected cells, ESCRT-III regulates INM integrity by downregulating excess INM.

PMC6107581 (PMC) (EuropePMC)