The following information accompanied stocks donated to the Bloomington Drosophila Stock Center by Joshua Mast and Nina DiPrimio, Perlara PBC.
TI{TI}CG3376KO - CRISPR/Cas9-mediated homologous dependent repair was used to replace the CG3376 coding region with a cassette carrying 3xP3-RFP, resulting in a null allele of CG3376. The cassette likely carries a single attP site and is flanked by loxP sites.
TI{TI}Npc1aKO - CRISPR/Cas9-mediated homologous dependent repair was used to swap a cassette carrying 3xP3-GFP for the Npc1a coding region. The result is a null allele of Npc1a. The cassette likely carries a single attP site and is flanked by loxP sites.
TI{TI}Pmm2KO - CRISPR/Cas9-mediated homologous dependent repair was used to swap a cassette carrying 3xP3-DsRed for the Pmm2 coding region. The result is a null allele of Pmm2. The cassette likely carries a single attP site and is flanked by loxP sites.
TI{TI}CG13397KO - CRISPR/Cas9-mediated homologous dependent repair was used to replace most of the CG13397 coding region (from -21 bp from the ATG to -91 bp from the stop codon) with a cassette carrying lox-P flanked 3xP3-RFP, resulting in a null allele of CG3376. The cassette also carries a single attP site.
TI{TI}CG14291KO - CRISPR/Cas9-mediated homologous dependent repair was used to replace most of the CG14291 coding region (from -93 bp from the ATG to -42 bp from the stop codon) with a cassette carrying lox-P flanked 3xP3-RFP, resulting in a null allele of CG3376. The cassette also carries a single attP site.