Mohr, M.A., Bushey, D., Aggarwal, A., Marvin, J.S., Kim, J.J., Marquez, E.J., Liang, Y., Patel, R., Macklin, J.J., Lee, C.Y., Tsang, A., Tsegaye, G., Ahrens, A.M., Chen, J.L., Kim, D.S., Wong, A.M., Looger, L.L., Schreiter, E.R., Podgorski, K. (2020). jYCaMP: an optimized calcium indicator for two-photon imaging at fiber laser wavelengths.  Nat. Methods 17(7): 694--697.

FBrf0246952

Research paper

Femtosecond lasers at fixed wavelengths above 1,000 nm are powerful, stable and inexpensive, making them promising sources for two-photon microscopy. Biosensors optimized for these wavelengths are needed for both next-generation microscopes and affordable turn-key systems. Here we report jYCaMP1, a yellow variant of the calcium indicator jGCaMP7 that outperforms its parent in mice and flies at excitation wavelengths above 1,000 nm and enables improved two-color calcium imaging with red fluorescent protein-based indicators.

PMC7335340 (PMC) (EuropePMC)