Luhur, A., Mariyappa, D., Klueg, K.M., Rogers, S.L., Zelhof, A.C. (2021). Serum-free adapted Drosophila S2R+ line is amenable to RNA interference. MicroPubl Biol 2021(): .
FlyBase ID
FBrf0248021
Publication Type
Research paper
Abstract
We have previously adapted a select number of Drosophila cell lines to grow in serum-free media supplemented with fly extract. This condition is arguably more representative of a native growth environment. Here, we validated that the fly extract adapted line, S2R+ (FEx 2.5%) is amenable to RNAi. RNAi against Rho1 in both S2R+ and S2R+ (FEx 2.5%) produced phenotypes similar to ones previously described in Drosophila S2 cells.
