Pavlova, G.A., Galimova, Y.A., Popova, Y.V., Munzarova, A.F., Razuvaeva, A.V., Alekseeva, A.L., Berkaeva, M.B., Pindyurin, A.V., Somma, M.P., Gatti, M., Renda, F. (2016). FACTORS GOVERNING THE PATTERN OF SPINDLE MICROTUBULE REGROWTH AFTER TUBULIN DEPOLYMERIZATION.  Tsitologiia 58(4): 299--303.

FBrf0250874

Research paper

We analyzed the pattern of spindle microtubule (MT) regrowth after cold- or colcemid-induced MT depolymerization in Drosophila S2 cells. Cold-induced MT disassembly at low temperature (–2 °C) destroyed kinetochore-driven MT regrowth without affecting astral MT formation. Conversely, colcemid-induced MT depolymerization strongly impaired centrosome-dependent MT nucleation, allowing kinetochore-driven MT regrowth. Collectively, these results indicate that the kinetochore- and the centrosome-mediated MT assembly pathways exploit molecular mechanisms that are at least in part different.