Wang, R., Yin, Y., Li, J., Wang, H., Lv, W., Gao, Y., Wang, T., Zhong, Y., Zhou, Z., Cai, Y., Su, X., Liu, N., Zhu, Z.J. (2022). Global stable-isotope tracing metabolomics reveals system-wide metabolic alternations in aging Drosophila.  Nat. Commun. 13(1): 3518.

FBrf0253789

Research paper

System-wide metabolic homeostasis is crucial for maintaining physiological functions of living organisms. Stable-isotope tracing metabolomics allows to unravel metabolic activity quantitatively by measuring the isotopically labeled metabolites, but has been largely restricted by coverage. Delineating system-wide metabolic homeostasis at the whole-organism level remains challenging. Here, we develop a global isotope tracing metabolomics technology to measure labeled metabolites with a metabolome-wide coverage. Using Drosophila as an aging model organism, we probe the in vivo tracing kinetics with quantitative information on labeling patterns, extents and rates on a metabolome-wide scale. We curate a system-wide metabolic network to characterize metabolic homeostasis and disclose a system-wide loss of metabolic coordinations that impacts both intra- and inter-tissue metabolic homeostasis significantly during Drosophila aging. Importantly, we reveal an unappreciated metabolic diversion from glycolysis to serine metabolism and purine metabolism as Drosophila aging. The developed technology facilitates a system-level understanding of metabolic regulation in living organisms.

PMC9209425 (PMC) (EuropePMC)