The following information accompanied stocks donated to the Bloomington Drosophila Stock Center by Carlos Lois, California Institute of Technology.
PBac{lexAop-nSyb-mCd19}
lexAop regulatory sequences drive expression of a fusion protein composed of the intracellular and transmembrane domains of nSyb, the extracellular domain of Mmus\Cd19 and the  Tag:OLLAS  tag.
P{elav-nlgSNTQF2}
elav regulatory sequences drives expression of 'nlgSNTQF2', an engineered receptor that can be used to monitor synaptic contacts between neurons in vivo. The nlgSNTQF2 protein is a fusion of 1. a single-chain antibody domain (SCAD) that recognizes the extracellular domain of Mmus\Cd19, 2. N amino acid residues 1460-1767, encoding the NRR (Notch regulatory region, a fragment of the extracellular domain) and TMD (transmembrane domain), 3. the  Tag:TS (Nlg2) tag (which results in the protein being targeted to postsynaptic sites) and 4. the QF2 driver. The coding sequence is also tagged at the C-terminal end with  Tag:V5 . When nlgSNTQF2 is expressed postsynaptically in one neuron type, and an appropriate ligand protein (containing the extracellular domain of Mmus\Cd19, the binding partner of SCAD) is expressed presynaptically on a neuron that forms a synaptic contact with the nlgSNTQF2-expressing neuron, then upon binding of nlgSNTQF2 and the Mmus\Cd19 fragment, components of the endogenous Notch/Delta signaling pathway cleave the N sequences in the NRR and TMD, releasing the QF2 driver, which can then enter the nucleus and activate transcription of a QUAS-driven construct.
PBac{UAS-nSyb.mCD19}
UAS regulatory sequences drive expression of a fusion protein composed of the intracellular and transmembrane domains of nSyb, the extracellular domain of Mmus\Cd19 and the  Tag:OLLAS  tag.
P{UAS-nSyb.mCD19}
UAS regulatory sequences drive expression of a fusion protein composed of the intracellular and transmembrane domains of nSyb, the extracellular domain of Mmus\Cd19 and the  Tag:OLLAS  tag.
P{QUAS-myr.mtdTomato}
QUAS regulatory sequences drive expression of the tdTomato fluorescent protein, tagged at the N-terminal end with a myristoylation signal.
P{QUAS-mtdTomato.PEST}
QUAS regulatory sequences drive expression of the tdTomato fluorescent protein, tagged at the C-terminal end with a PEST degradation signal, with the aim of reducing the half-life of the protein.
P{UAS-CD4-tdGFP}2        is a second chromosome insertion.
P{nSyb-nlgSNTG4}attP2 is an insertion at the P{CaryP}attP2 site, 3L(68A4).
PBac{lexAop-nSyb-mCd19}VK00027        is an insertion at the PBac{y+-attP-9A}VK00027 site, 3R (89E11).
P{elav-nlgSNTQF2}M6    is a second chromosome insertion.
P{elav-nlgSNTQF2}M3    is a third chromosome insertion.
PBac{UAS-nSyb.mCD19}VK00022 is an insertion at the the PBac{y+-attP-9A}VK00022 site, 2R(57F5)
P{UAS-nSyb.mCD19}attP2           is an insertion at the P{CaryP}attP2 site, 3L(68A4).
P{QUAS-myr.mtdTomato}14       is a third chromosome insertion.
P{QUAS-mtdTomato.PEST}M1   is a third chromosome insertion, homozygous viable.