The following information accompanied stocks donated to the Bloomington Drosophila Stock Center by Bing Ye, University of Michigan, Ann Arbor.
P{UAS(FRT.rCD2-stop) CD4::tdTomato }2 is a homozygous viable insertion on the second chromosome.
P{UAS(FRT.rCD2-stop) mCD8::GFP }2 is an insertion on the second chromosome.
P{UAS(FRT.rCD2-stop) mCD8::GFP }3 is a homozygous viable insertion on the third chromosome.
P{10xUAS-IVS-Syn21-cAMPFIRE-M-p10} expresses the cAMPFIRE-M cAMP sensor under the control of 10xUAS sequences (generated by replacing the GFP in FBtp0073107 with the sensor sequence).
P{10xUAS-IVS-Syn21-cAMPFIRE-H-p10} expresses the cAMPFIRE-H cAMP sensor under the control of 10xUAS sequences (generated by replacing the GFP in FBtp0073107 with the sensor sequence).
P{10xUAS-IVS-Syn21-cAMPFIRE-M-dark-p10} expresses the cAMPFIRE-M-dark cAMP sensor under the control of 10xUAS sequences (generated by replacing the GFP in FBtp0073107 with the sensor sequence).
P{10xUAS-IVS-Syn21-cAMPFIRE-H-dark-p10} expresses the cAMPFIRE-H-dark cAMP sensor under the control of 10xUAS sequences (generated by replacing the GFP in FBtp0073107 with the sensor sequence).
Dark cAMPFIREs are cAMPFIRE sensors that use dark tdVenus as the acceptor. Dark tdVenus provides two advantages: 1) since mTurquoise2 and tdVenus spectrum overlap, a dark tdVenus allows the collection of broader bandwidth of mTurquoise2 spectrum. 2) Dark tdVenus reduces the bleedthrough signal at the orange and red spectrum, facilitating the multiplexed use of a sensor or cytosolic marker at those wavelengths.
P{10xUAS-IVS-Syn21-mTurquoise2-p10} expresses mTurquoise2 under the control of 10xUAS sequences (generated by replacing the GFP in FBtp0073107 with mTurquoise2).
P{10xUAS-IVS-Syn21-td-cpVenus-p10} expresses two tandem copies of a circularly permuted version of Venus under the control of 10xUAS sequences (generated by replacing the GFP in FBtp0073107 with td-cpVenus).
The following are insertions on the second chromosome into attP40 (FBti0114379):
P{10xUAS-IVS-Syn21-cAMPFIRE-M-p10}attP40
P{10xUAS-IVS-Syn21-cAMPFIRE-H-p10}attP40
P{10xUAS-IVS-Syn21-cAMPFIRE-M-dark-p10}attP40
P{10xUAS-IVS-Syn21-cAMPFIRE-H-dark-p10}attP40
P{10xUAS-IVS-Syn21-mTurquoise2-p10}attP40
P{10xUAS-IVS-Syn21-td-cpVenus-p10}attP40
PBac{UAS-dock.OLLAS}VK00019 is an insertion on the third chromosome into VK00019 (FBti0076440).