Using the Drosophila melanogaster genomic region r6.52 as a reference: PEK1 has a single bp deletion of a “C” at 3R:5462724 . PEK2 has a 14-bp deletion of “GTGGCCCCGGTCCG” at 3R:5462712..5462725 . PEKΔLD has a 2902-bp deletion at 3R:5461414..5464315 and is replaced by a 3xHA sequence. It also has silent mutations from “GG” to “AT” at 3R:5460966..5460967 + to mutate the gRNA PAM sequence. See the associated files with sequence and chromatograms for PEKΔLD, and chromatograms for homozygote and heterozygote PEK1, PEK2 and wt PEK.