The following information accompanied stocks donated to the Bloomington Drosophila Stock Center by Yihong Ye, NIH, National Institute of Diabetes and Digestive and Kidney Diseases.
P{UAS-ER.GFP.K0} expresses a signal sequence for co-translational ER targeting, a N-glycosylation site (-CHO) followed by GFP, FLAG tags (no lysine residues have been added to alter ribosome elongation) and mCherry, under UAS control.
P{UAS-ER.GFP.K0}attP2 is a viable and fertile insertion on the third chromosome into attP2 (FBti0040535).
PBac{UAS-hSNCA.mKeima} expresses human alpha synuclein tagged with pH sensor mKeima under UAS control.
PBac{UAS-hSNCA.mKeima}VK00037 is a viable and fertile insertion on the second chromosome into VK00037 (FBti0076455).
P{UAS-mKeima.Y} expresses pH-sensitive fluorescent protein mKeima under UAS control.
P{UAS-mKeima.Y}attP2 is a viable and fertile insertion on the third chromosome into attP2 (FBti0040535).
PBac{UAS-hSNCA.mCherry} expresses human alpha synuclein tagged with mCherry under UAS control.
PBac{UAS-hSNCA.mCherry}VK00037 is a viable and fertile insertion on the second chromosome into VK00037 (FBti0076455).
PBac{UAS-hSNCA.A30P.mCherry} expresses human alpha synuclein with A30P mutation tagged with mCherry under UAS control.
PBac{UAS-hSNCA.A30P.mCherry}VK00037 is a viable and fertile insertion on the second chromosome into VK00037 (FBti0076455).
PBac{UAS-hSNCA.A53T.mCherry} expresses human alpha synuclein with A53T mutation tagged with mCherry under UAS control.
PBac{UAS-hSNCA.A53T.mCherry}VK00037 is a viable and fertile insertion on the second chromosome into VK00037 (FBti0076455).