FB2026_03 , released September 17, 2026
Sequence Feature: Dmel\DRSC33315
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General Information
Symbol
Dmel\DRSC33315
Species
D. melanogaster
Feature type
FlyBase ID
FBsf0000030159
Genomic Location
Chromosome (arm)
2L
Sequence location

2L:21,418,512..21,418,649 [+]

2L:21,472,818..21,472,955 [+]

2L:21,467,775..21,467,912 [+]

2L:21,462,732..21,462,869 [+]

2L:21,457,672..21,457,809 [+]

2L:21,452,611..21,452,748 [+]

2L:21,438,570..21,438,707 [+]

2L:21,433,522..21,433,659 [+]

2L:21,428,475..21,428,612 [+]

2L:21,423,414..21,423,551 [+]

Sequence Data
Length
138
Comments

One copy of repeat shown

GTGTCAGGATGGACCTGCTTGAGAACCTTGTAAATGTAGATGGCATAGCTCTCCTTCCTTTTGCGCTTCTTTTTCTTGTC
GGTCTTGGTGATGTTCTTCTGAGCCTTGCCAGCCTTCTTGGCTGCCTTTCCACTAGTT
Associated Information
Experimental Data
Progenitors
PCR template

genomic DNA

Primer 1
AACTAGTGGAAAGGCAGCCA
Primer 2
GTGTCAGGATGGACCTGCTT
Comments
Collection Information
Collection: DRSC_dsRNA
Symbol
Title
DRSC collection of dsRNA for RNAi in cell culture.
Source and Progenitors
Species of derivation
D. melanogaster
Strain of derivation
Vector of progenitor construct
Description

Comprehensive collection of double-strand RNA fragments (1-2 dsRNAs per annotated gene), used for genome-wide RNAi-knockdown assays in cell culture.

Additional data
Sample preparation
Collection preparation

The DRSC RNAi screening library was constructed from genomic DNA amplified using Oregon-R strain. In initial collection, most amplicons were designed to FlyBase rel2 at R.Paro's lab (ZMBH Heidelberg). Primers were selected to target the open reading frames (ORF) of known and predicted genes. Regularly updated to accommodate newly identified genes and new gene models, and to replace amplicons with high potential for off-target effects.

Mode of assay
Data analysis
Cell lines
Observed in
Not observed in
Comments
Stocks (0)
External Crossreferences and Linkouts ( 1 )
Crossreferences
DRSC - Results frm RNAi screens
Synonyms and Secondary IDs (1)
Reported As
Symbol Synonym
Secondary FlyBase IDs
  • FBri0029808
References (4)