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General Information
Symbol
Dmel\P{GawB}pathc135
Species
D. melanogaster
Name
FlyBase ID
FBti0002589
Also Known As
C135-GAL4, c135, P{GawB}c135
Description
Inserted Element
Uses

GAL4 reporter/driver

Affected gene(s)
Viability / fertility
viable
Associated allele(s)
Stock availability
LINE ID
Genomic Location
Chromosomal Location
3L ( 67B10 )
Sequence Location
3L:9,504,820..9,504,820
Target / Docking site
Member of Large Scale Dataset(s)
Dataset
Description
Detailed Mapping Data
Chromosome (arm)
Sequence Location
3L:9,504,820..9,504,820
Orientation
Cytological location (computed by FlyBase)
67B10 (inferred by FlyBase from sequence location)
Cytological location (reported)
Insertion into Natural transposon
Comments concerning location

The insertion was mapped to 5642bp upstream of the path translation start site.

Sequence Data
Flanking sequence
Inserted Element
Construct
Location-dependent role
Size (Kb)

11.279

Associated alleles
Molecular map
Affected Gene(s)
Alleles and Phenotypes
Expression Data
Reporter Expression
distribution deduced from reporter (Gal4 UAS)
Stage
Tissue/Position (including subcellular localization)
Reference
Additional Information
Statement
Reference

Scer\GAL4path-c135 drives strong expression in carpet glial cells of the eye disc in third instar larvae; this expression is gradually diminished during pupal development.

Expressed in embryonic proventriculus, larval brain, eye disc, gut, fat body, adult ovarian squamous and posterior terminal follicle cells, male accessory glands, seminal vesicles, ejaculatory duct, cyst cells and spermatocytes.

Expression in the midgut is found in a distinct group of Dh31-expressing enteroendocrine cells located at the junction region of the anterior midgut and the acid-secreting portion of the larval midgut. This junction region occurs at a conspicuous and characteristic U-shaped bend in the gut. These are referred to as the "midgut junction Dh31-expressing cells".

During oogenesis, Scer\GAL4path-c135 drives expression in the follicle cells at the posterior of the egg chamber at oogenesis stage 9-10, and in squamous nurse follicle cells and in centripetally migrating follicle cells at stage 10, and in posterior terminal cells. It does not drive expression in oocyte associated follicle cells. In the male reproductive tract, Scer\GAL4path-c135 drives expression in the male accessory gland secondary secretory cells, the seminal vesicle, the ejaculatory duct, cyst cells, and spermatocytes. In embryos, Scer\GAL4path-c135 drives expression in a ring-shaped pattern in the proventriculus. At larval stages, Scer\GAL4path-c135 drives expression in the brain, in glial cells in the eye disc, in the gut, and in the fat bodies.

Expression during embryogenesis has not yet been described. However, when used to drive expression of UAS constructs that are usually lethal when expressed in embryos, animals can be recovered. This suggests there is little or no embryonic expression.

Expression in the lymph gland is uniform.

 
Reflects expression of
Data on Genetic Line
Line ID
Origin as a multiple insertion line
Progenitor(s) within the Genome
Related Aberration or Balancer
Aberration
Balancer
Stocks (2)
External Crossreferences and Linkouts ( 0 )
Comments
Comments

The orientation of the insertion was not reported.

Generated by K. Kaiser.

When directing expression of P{UAS-hop.Tum} or P{UAS-hop.H}, capable of producing melanotic tumors in larvae.

Synonyms and Secondary IDs (12)
References (21)