FlyBase Insertion Report: Dmel\P{PZ}dac[P]

General Information

Symbol

Dmel\P{PZ}dac^P

Species

D. melanogaster

Name

FlyBase ID

FBti0003463

Feature type

transposable_element_insertion_site

Also Known As

dac-lacZ

Description

Inserted Element

P{PZ}

Uses

lacZ reporter

Affected gene(s)

dac, Ecol\lacZ

Viability / fertility

Associated allele(s)

dac^P, Ecol\lacZ^dac-P

Stock availability

1 publicly available

LINE ID

l(2)rK364

Genomic Location

Chromosomal Location

2L ( 36A1 )

Sequence Location

2L:16,485,982..16,485,982 [+]

Target / Docking site

Genomic Maps

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Member of Large Scale Dataset(s)

Dataset

TI_set_P{PZ}.BDGP

Description

Insertion lines from this collection were assessed for inclusion in the Gene Disruption Project collection.

(Bellen et al., 2004)

A set of mutant stocks derived by insertional mutagenesis using the P-element construct P{PZ}; most lines have a lethal or sterile phenotype. The P{PZ} construct carries a ry[+] visible marker, Ecol\lacZ enhancer trap sequences, and bacterial sequences that allow plasmid rescue.

(Spradling et al., 1999)

Detailed Mapping Data

Chromosome (arm)

Sequence Location

2L:16,485,982..16,485,982 [+]

(BDGP Project Members, 1994-1999)

Orientation

Cytological location (computed by FlyBase)

36A1 (inferred by FlyBase from sequence location)

Cytological location (reported)

36A1-36A2 (in situ hybridization reported)

(BDGP Project Members, 1994-1999)

Insertion into Natural transposon

Comments concerning location

Sequence Data

Flanking sequence

AQ026135

Inserted Element

Construct

P{PZ}

Location-dependent role

Size (Kb)

14.545

Associated alleles

l(3)87Df^7.2

ry^+t7.2

Molecular map

Affected Gene(s)

Insertion may affect gene

dac

(Mardon et al., 1994)

Ecol\lacZ

(Ahn et al., 2011, Chu et al., 2002, Dong et al., 2002, Galindo et al., 2002, Martini et al., 2000, Erkner et al., 1999, Wu and Cohen, 1999, Abu-Shaar and Mann, 1998, Couso and Bishop, 1998, Mardon et al., 1994)

Alleles and Phenotypes

Allele

dac^P

Reference(s)

(Chu et al., 2002, Martini et al., 2000, Ashburner et al., 1999, Ashburner et al., 1999.10.12, Spradling et al., 1999, BDGP Project Members, 1994-1999, Mardon et al., 1994)

Phenotype Data

Phenotypic Class Phenotype Manifest in Detailed Description

Allele

Ecol\lacZ^dac-P

Reference(s)

Phenotype Data

Phenotypic Class Phenotype Manifest in Detailed Description

Expression Data

Reporter Expression

distribution deduced from reporter

Stage

Tissue/Position (including subcellular localization)

Reference

wandering third instar larval stage

imaginal disc

(Meister and Braun, 1995.10)

adult stage

calyx of adult mushroom body

(Martini et al., 2000)

Additional Information

Statement

Reference

In third instar larva, expression in imaginal discs (which discs not specified).

(Meister and Braun, 1995.10)

Marker for

Reflects expression of

dac

(Martini et al., 2000)

Reporter construct used in assay

Data on Genetic Line

Line ID

l(2)rK364

(Gene Disruption Project members, 2001-)

Origin as a multiple insertion line

Progenitor(s) within the Genome

Related Aberration or Balancer

Aberration

Balancer

Stocks (1)

Bloomington

12047

P{PZ}dac^P/CyO; ry⁵⁰⁶

External Crossreferences and Linkouts ( 0 )

Comments

Location 2L:16481911-16481912 confirmed by FlyBase alignment of dbGSS accession AQ026135 to D. melanogaster arm Release_4 and heterochromatin Release_3.2b.

(FlyBase, 2005)

Synonyms and Secondary IDs (10)

Reported As

Symbol Synonym

P2047

(Meister and Braun, 1995.10)

P{PZ}dac^P

(FlyBase, 2005, Mardon et al., 1994, FlyBase, 1992-)

P{PZ}dac^rK364

(FlyBase, 1992-)

P{PZ}l(2)rK364^rK364

(FlyBase, 1992-)

P{PZ}rK364

(Ashburner et al., 1999)

P{ry^+t7.2=PZ}dac^rK364

(FlyBase, 1992-)

P{ry^+t7.2=PZ}l(2)rK364^rK364

(FlyBase, 1992-)

dac-lacZ

(Ahn et al., 2011, Chu et al., 2002, Dong et al., 2002, Wu and Cohen, 1999)

dac¹⁰lacZ

(Galindo et al., 2002)

dacLacZ

(Erkner et al., 1999)

Name Synonyms

Secondary FlyBase IDs

FBti0000455
FBti0003024

References (22)

Report Sections

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