FB2020_06, released Dec 22, 2020

Insertion: Dmel\P{PZ}ms(2)35Ci02316

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General Information
Symbol
Dmel\P{PZ}ms(2)35Ci02316
Species
D. melanogaster
Name
FlyBase ID
FBti0003526
Feature type
transposable_element_insertion_site
Description
Inserted Element
P{PZ}
Uses

lacZ reporter

Affected gene(s)
Ecol\lacZ, ms(2)35Ci
Viability / fertility
Associated allele(s)
Ecol\lacZms(2)35Ci-02316, ms(2)35Ci02316
Stock availability
2 publicly available
LINE ID

l(2)02316

ms(2)02316

Genomic Location
Chromosomal Location
2L ( 35D1 )
Sequence Location
2L:15,293,209..15,293,209 [+]
Target / Docking site
Genomic Maps
Member of Large Scale Dataset(s)
Dataset
TI_set_P{PZ}.BDGP
Description

Insertion lines from this collection were assessed for inclusion in the Gene Disruption Project collection.

(Bellen et al., 2004)

A set of mutant stocks derived by insertional mutagenesis using the P-element construct P{PZ}; most lines have a lethal or sterile phenotype. The P{PZ} construct carries a ry[+] visible marker, Ecol\lacZ enhancer trap sequences, and bacterial sequences that allow plasmid rescue.

(Spradling et al., 1999)
Detailed Mapping Data
Chromosome (arm)
Sequence Location
Orientation
Cytological location (computed by FlyBase)
35D1 (inferred by FlyBase from sequence location)
Cytological location (reported)
46A1-46B14 (in situ hybridization reported)
(BDGP Project Members, 1994-1999)
Insertion into Natural transposon
Comments concerning location

Initial data, including cytological localization by in situ hybridization, conflict with genomic sequence-based localization; this may indicate that there was a second insertion in the original line.

(FlyBase, 1996-)
Sequence Data
Flanking sequence
AQ026404
(FlyBase, 1992-)
Inserted Element
Construct
P{PZ}
Location-dependent role
lacZ enhancer trap
(Mlodzik and Hiromi, 1992)
Size (Kb)

14.545

Associated alleles
l(3)87Df7.2
ry+t7.2
Molecular map
Affected Gene(s)
Alleles and Phenotypes
Expression Data
Reporter Expression
distribution deduced from reporter
Stage
Tissue/Position (including subcellular localization)
Reference
wandering third instar larval stage
imaginal disc
(Meister and Braun, 1995.10)
Additional Information
Statement
Reference

In wandering third instar larva, weak (uncertain) expression in imaginal discs (which discs not specified).

(Meister and Braun, 1995.10)
 
Marker for
Reflects expression of
Reporter construct used in assay
Data on Genetic Line
Line ID

ms(2)02316

(Gene Disruption Project members, 2001-)

l(2)02316

(Gene Disruption Project members, 2001-)
Origin as a multiple insertion line

p

(FlyBase, 1996-)
Progenitor(s) within the Genome
Related Aberration or Balancer
Aberration
Balancer
Stocks (2)
External Crossreferences and Linkouts ( 0 )
Comments
Comments

Location 2L:15289138-15289139 confirmed by FlyBase alignment of dbGSS accession AQ026404 to D. melanogaster arm Release_4 and heterochromatin Release_3.2b.

(FlyBase, 2005)
Synonyms and Secondary IDs (6)
Reported As
Symbol Synonym
P1759
(Meister and Braun, 1995.10)
P{PZ}02316
(Ashburner et al., 1999)
P{PZ}ms(2)35Ci02316
(FlyBase, 2005, FlyBase, 1992-)
P{PZ}ms(2)46AB02316
(Castrillon et al., 1993, FlyBase, 1992-)
P{ry+t7.2=PZ}ms(2)46AB02316
(FlyBase, 1992-)
l(2)02316
(FlyBase, 1992-)
Name Synonyms
Secondary FlyBase IDs
  • FBti0000702
References (13)